Fig. 2: Immune cell phenotyping and associations between mucinous differentiation and immune cell densities.

a pseudo-immunofluorescence images for visualising three multiplex immunohistochemistry staining panels. b Cell phenotyping maps for immune cell subtypes, tumour cells, and other cells. c Associations between mucinous differentiation and immune cell densities in MMR-proficient and deficient tumours. P values were determined using the Wilcoxon rank-sum test. Statistically significant correlations are shown with asterisks (***P < 0.0001; **P < 0.001; *P < 0.005). Immune cell density analyses for MMR-proficient and deficient tumours are based on 870 and 156 cases (T cells, macrophages, M1-like macrophages, M2-like macrophages), 873 and 159 cases (B cells, plasma cells), 856 and 152 cases (mature monocytic cells, immature monocytic cells, granulocytes, mast cells), respectively. MMR, mismatch repair.