Fig. 2: Exploration of key bacteria and bacterial metabolites affecting the efficacy of IRT.

a Cluster tree analysis of multiple samples. b PCA analysis of multiple samples in metagenomics. c Circos plot of differential analysis across multiple groups at the family level. d Bar plot of differential analysis across multiple groups at the genus level. e Relative abundance of Bacteroidaceae. f Relative abundance of Lachnospiraceae. g Relative abundance of Clostridiaceae. h Relative abundance of fatty acid metabolism. i Relative abundance of fatty acid biosynthesis. j Relative abundance of fatty acid degradation. Concentration of acetic acid (k), propionic acid (l), butyric acid (m), isobutyric acid (n), valeric acid (o), isovaleric acid (p), and caproic acid (q) in intestinal contents. r Correlation analysis between SCFAs and gut bacteria. ***p < 0.001, **p < 0.01, *p < 0.05, ns not significant.