Fig. 5: Presence of MDM associates with positive ICI response and prolonged survival. | British Journal of Cancer

Fig. 5: Presence of MDM associates with positive ICI response and prolonged survival.

From: Anti-PD-1 treatment response is associated with the influx of circulating myeloid and T-cell subsets into the metastatic melanoma tumor microenvironment

Fig. 5

a Violin plots illustrating the Z-scores of the ‘monocyte-derived-macrophage’ (MDM) signature [31] in tumors of non-responders (NR) and responders (R) prior to anti-PD-1 treatment [30]. Mann-Whitney U-test was applied. b Kaplan–Meier curves demonstrate overall survival (OS, left) and progression-free survival (PFS) (right) in tumor subgroups of anti-PD-1 pre-treatment samples of metastatic melanoma patients characterized by the MDM signature using the k-means algorithm. A two-sided log-rank test was applied. c Violin plots illustrate the messenger RNA (mRNA) expression (log2-transformed RPM) of immune checkpoint-related genes (PD-1 (PDCD1), PD-L1 (CD274), SIRPα (SIRPA), CD47 (CD47), and VISTA (VSIR) in MDMHigh and MDMLow tumors of metastatic melanoma patients prior to anti-PD-1 treatment. Mann-Whitney U-test was applied. d UMAP of single immune cell transcriptome profiles prior to anti-PD-1 treatment from metastatic melanoma patients (5 responders, 11 non-responders) [29]. e Violin plot illustrating the Z-scores of the MDM signature [31] in monocytes and macrophages prior to anti-PD-1 treatment. Mann-Whitney U-test was applied. f Proportion of MDMLow and MDMHigh (characterized using the k-means algorithm) in monocytes and macrophages. Chi-squared test was applied. g Violin plots illustrate the mRNA expression (log2-transformed RPM) of immune checkpoint-related genes (PD-1 (PDCD1), PD-L1 (CD274), SIRPα (SIRPA), CD47 (CD47), and VISTA (VSIR) in monocytes, macrophages, CD4+ T cells, and CD8+ T cells. h Violin plots illustrate the mRNA expression (log2-transformed RPM) of cytokines and chemokine genes (CSF1, CSFR1, CCL2, CCL5, CCR7, IL34, CXCL17, CX3CL1, CXCL9, CXCL10, CXCL11, CXCL13) in MDMHigh and MDMLow tumors of metastatic melanoma patients prior to anti-PD-1 treatment [30]. Mann-Whitney U-test was applied. i. Violin plots illustrate the mRNA expression (log2-transformed RPM) of cytokines and chemokine genes (CSF1R, CCL2, CCL5, CCR7, CX3CR1, CXCR5, CXCL13, CXCR3, CXCL9, CXCL10, CXCL11, CCR2, CCR4, CCR5) in monocytes, macrophages, CD4+ T cells, and CD8+ T cells [29].

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