Fig. 3: BI-D1870 reduces PM cell viability.
a Viability of PM cells treated with BI-D1870 at the indicated concentrations for 72 h, determined by a SYBR green-based growth assay. b IC50 values of BI-D1870 after 72 h, calculated from dose-response curves in correlation with cell viability 96 h after transfection with 5 nM of YB-1-specific siRNA compared to control siRNA (left), and YB-1 expression levels normalised to the immortalised mesothelial cell line Met-5A assessed by immunoblot. Each dot represents one cell line and is the mean of at least 3 biological replicates. Pearson correlation, *p < 0.05, **p < 0.005. c Representative images and d quantification of colony formation assays treated with DMSO (Co) or 5 µM and 15 µM BI-D1870 (BI) stained with crystal violet after 7–14 days. The data are shown as mean + SEM. ***p < 0.001. Scale bar = 1 cm.
