Fig. 1: Human colon cancer organoid culture in human-tissue-derived decellularized ECM.
From: Single-cell multiomic modelling of early metastatic events promoted by the extracellular matrix
a Overall scheme of our research. Human colon cancer organoids were cultured both in the BME and pdECM. Cultured organoids were analyzed by single-cell multiomic sequencing. b Flow chart of our organoid culture involving matrix transition. c Organoid growth in the BME and pdECM by measuring relative covered area. Standard deviation was expressed in the graph. d Representative images of organoids cultured in the BME and pdECM. Scale bar. 100 μm. e Representative images of nucleus and actin filament of BME-O and pdECM-O. Scale bar. 100 μm. a–e BME basement membrane extract, BME-O BME-cultured organoid, pdECM patient-derived extracellular matrix, pdECM-O pdECM-cultured organoid.
