Fig. 5: Single-cell transcriptomic atlas of GC patients reveals the expression landscape of VSIR across distinct cell populations.

a Single-cell transcriptomic atlas of GC patients, showing distinct cell clusters including T&NK cells, B cells, plasma cells, myeloid cells, mast cells, endocrine cells, epithelial cells, endothelial cells, fibroblasts, and smooth muscle cells. b UMAP dimensionality reduction plots of myeloid cells subpopulations and the classification of various subsets. c VSIR expression mapped onto UMAP. Blue circles: high VSIR expression in myeloid cells. d The expression levels of VSIR across different cellular subpopulations were compared, revealing that its expression in myeloid cells was significantly higher than in other subpopulations. e Expression levels of VSIR in different myeloid cell populations, with the median values annotated in the bar chart. F Different subsets of Monocyte-Macrophage cells exhibit distinct characteristic M1/M2/antigen presentation functional genes. g Functional scores of multiple gene sets in monocyte-macrophage populations, calculated using UCell. h Comparison of gene set functional scores between VSIR⁺ and VSIR^- cells in monocyte-macrophage subpopulations. *P < 0.05, **P < 0.01, ***P < 0.001.