Fig. 3: A—Imbalance Assay for EML4::ALK fusion.
From: Diagnostic whole transcriptome sequencing in a series of 1233 FFPE solid tumor samples
Top: IGV representation of the missed EML4::ALK fusion in WTS: +/- represent the positive/negative strand based on stranded protocol information. Spliced reads for ALK on the + strand are depicted as grey lines connecting the grey cigars, spliced reads of the—strand are depicted in blue. The splice junction track on top represents splice junctions on the + strand in blue and on the—strand in red. Unspliced reads originating from CLIP4 reaching into the kinase domain of ALK on the—strand. Bottom: Visualization of the EML4::ALK fusion with the coverage track on top, the exon and breakpoint representation below and the resulting fusion transcript on bottom.
