Fig. 4: PAKi treatment sensitises HGSC cells to activated CD8+ T cell killing.

Plots show MTT viability results of (a) Ovsaho and (b) Kuramochi cells after PAKi concurrent treatment and PAKi pre-treatment followed by co-culture with PBMC isolated CD8+ T cells at 1:4 (T:E). Statistical significance was calculated with one-way ANOVA and post hoc analyses comparing untreated cells with other experimental conditions unless otherwise indicated: ns, not significant, p > 0.05; *p < 0.05; **p < 0.01; ***p < 0.001. Plots show PD-L1 surface expression on (c) Ovsaho and (d) Kuramochi cells after 48 h co-culture with activated CD8+ T cells, with an increase in surface PD-L1 in stained populations (blue peaks) compared to isotype control populations (red peaks). All figures represent N = 3 biologically independent experiments.