Table 1 Primary antibodies.

From: Investigating PAK inhibition in combination with PD-1 blockade to enhance cytotoxic CD8+ T cell-mediated killing and suppress invasion of ovarian cancer cells

Primary antibodies

Antibody

Company

Catalogue number

Source

WB dilution

β-tubulin

Protein Tech

66240-1-1g

Mouse

1:2000

GAPDH

Santa Cruz Biotechnology

MAB374

Mouse

1:2000

Hsp90

Santa Cruz Biotechnology

SC-13119

Rabbit

1:1000

PAK1

Cell Signaling

2602S

Rabbit

1:1000

PAK2

Cell Signaling

2608S

Rabbit

1:1000

PAK4

Cell Signaling

62690S

Rabbit

1:2000

EGFR

Cell Signaling

4267S

Rabbit

1:1000

PD-L1, clone 5H1

Millipore

MAB1115

Mouse

1:1000

PAK3

Cell Signaling

2609S

Rabbit

1:1000

PAK5 (PAK7)

Protein Tech

66961-1-1g

Mouse

1:1000

PAK6

Gene Tex

GTX127915

Rabbit

1:1000

HER2 (29D8)

Cell Signaling

2165

Rabbit

1:1000

HER4 (111B2)

Cell Signaling

4795

Rabbit

1:500

β-Catenin (D10A8) XP®

Cell Signaling

8480S

Rabbit

1:1000

pβ-catenin (S675)

Cell Signaling

9567S

Rabbit

1:1000

Akt (pan) (C67E7)

Cell Signaling

C67E7

Rabbit

1:1000

pAKT (S473)

Cell Signaling

9271S

Rabbit

1:1000

MAPK (ERK1/ERK2)

Cell Signaling

9102S

Rabbit

1:1000

Phospho-p44/42 MAPK (ERK1/ERK2) (Thr202/Tyr204)

Cell Signaling

9106S

Mouse

1:2000

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