Fig. 2: Expression of various markers in 3D-UHU-TU. | British Journal of Cancer

Fig. 2: Expression of various markers in 3D-UHU-TU.

From: An advanced in vitro bladder cancer model integrating bladder cancer spheroids into a healthy human urothelium for preclinical therapeutic testing

Fig. 2: Expression of various markers in 3D-UHU-TU.The alternative text for this image may have been generated using AI.

a E- and N-Cadherin (grey) in 3D-UHU-TU in 100% urine environment 2 days post-implantation (3D-UHU layer below plane of focus). For merged images, Cadherin = Red, nuclei = cyan. Scale bars = 50 µm. b H&E and IHC in models maintained for 6 days in 100% urine environment. Images are representative of 3 separate biological replicates. Bars = 50 µm. c CK20 expression (left panels; greyscale) with 3D merges. Models were stained for F-Actin (magenta), which highlights the actin cytoskeleton, useful for providing an overview of cell morphology, nuclei (cyan), cancer spheroid (green) and CK20 (yellow). CK20 on urothelium are highlighted with white arrows. Models maintained for 6 days in 100% urine environment. d Migration phenotypes magnified from Fig. S2B, C. MMP-2 & -9 (red) and nuclei (cyan) stained. Scale bars = 10 µm. Representative images from 3 independent experiments.

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