Fig. 5: oHSV-GFP treatment of 3D-UHU-TU models. | British Journal of Cancer

Fig. 5: oHSV-GFP treatment of 3D-UHU-TU models.

From: An advanced in vitro bladder cancer model integrating bladder cancer spheroids into a healthy human urothelium for preclinical therapeutic testing

Fig. 5: oHSV-GFP treatment of 3D-UHU-TU models.The alternative text for this image may have been generated using AI.

a Specific co-localisation of oHSV-GFP with RT112 and T24 cancer spheroids with minimal to no viral infection in healthy 3D-UHU component. oHSV-GFP propagated in cancer spheroids over 72 h post treatment (PT). Merged images are shown as summed z-projects. Scale bars = 20 µm. b Percentage cytotoxicity of 3D-UHU-TU models over 120 h PT. *P = < 0.05. Data are presented as mean averages of 3 independent experiments for 96 and 120 h and 2 independent experiments for 72 h. c Syncytia were also observed after 72 h PT in both cancer spheroids typical of fusogenic viruses. Scale bars = 20 µm. Images are representative of 3 biological replicates unless stated. For all merged images, nuclei=blue; F-actin=red; oHSV-GFP=Green. Cancer spheroid=greyscale (shown separately).

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