Fig. 4
From: Increasing the bactofection capacity of a mammalian expression vector by removal of the f1 ori
Stress response activation in S. Typhimurium carrying pEGFP. Cultures were grown to mid-log phase (OD600 of ~0.6) and harvested for western blot analysis for stress response proteins or immunofluorescence staining. a Immunoblot analysis for RecA across multiple wild type or pEGFP-transformed SL7207 colonies with mitomycin C-treated (Mito) wild type SL7207 cultures serving as a positive control for RecA activation. RecA and GroEL levels were determined in at least three independent replicates of SL7207 and SL-pEGFP. b Representative DAPI-stained wild type and SL-pEGFP cultures. Images were pseudo-coloured to aid visualisation of the segmented filamentous phenotype of SL-pEGFP. Scale bars 5 µm. Representative DAPI-stained images are shown for each culture
