Table 2 Plasmids used in this study
From: Increasing the bactofection capacity of a mammalian expression vector by removal of the f1 ori
Plasmid | Function | Features | Source |
|---|---|---|---|
pEGFP-C2 | Eukaryotic expression vector | EGFP under the control of the CMV promoter | Clontech |
pUC19 | High copy number plasmid | pUC19 origin of replication | NEB |
Source of lacZ gene | lacZ gene | ||
pLuc | Eukaryotic expression vector similar to pEGFP | Ampicillin resistance, luciferase transgene, f1 ori | Addgene, 45968 |
prpsM-GFP | Prokaryotic GFP reporter plasmid | Constitutive prokaryotic GFP expression | [66] |
pEGFP(-f1) | Test if removal of f1 ori from pEGFP abrogates filamentation | pEGFP lacking f1 ori | This study |
pACYC-EGFP | Test if EGFP can drive filamentation in pACYC184 | rpsmGFP plasmid plus EGFP transgene from pEGFP | This study |
