Fig. 1: Identification and characterisation of FRα binding oligopeptides from phage display libraries. | Cancer Gene Therapy

Fig. 1: Identification and characterisation of FRα binding oligopeptides from phage display libraries.

From: Identification of folate receptor α (FRα) binding oligopeptides and their evaluation for targeted virotherapy applications

Fig. 1

a Characterisation of FRα expression on SKOV3 cells. b Phage peptide recovery from SKOV3 cells eluted with folic acid. Cell binding of homogeneous selected phage clones recovered from SKOV3 cells eluted with folic acid, c fold change relative to insert-less phage. Bars represent the mean ± S.D. #P ≤ 0.05 versus DRLADGV (non-consensus) control peptide. *P ≤ 0.05 versus insert-less control peptide. d Phage clones bind SKOV3 cells in vitro. 7-mer cyclic phage peptides (CTVRTSAEC, CIGNSNTLC) and 12-mer linear peptides (DWSSWVYRDPQT) bind to SKOV3 cells. Cells were infected with 2 × 1010 pfu of phages at 4 °C and detected by anti-M13 antibody. An empty (insert-less) phage clone was used as a control.

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