Fig. 2: Evaluation and competitive inhibition of TVRTSAE peptide binding to FRα on SKOV3 cells. | Cancer Gene Therapy

Fig. 2: Evaluation and competitive inhibition of TVRTSAE peptide binding to FRα on SKOV3 cells.

From: Identification of folate receptor α (FRα) binding oligopeptides and their evaluation for targeted virotherapy applications

Fig. 2

a Synthesised TVRTSAE peptide binding to SKOV3 cells increases with escalating peptide concentration. SKOV3 cells were incubated with increasing peptides concentration (100, 300 or 500 µM) for 1 h at 4 °C and analysed by flow cytometry. PBS was used as a no-peptide control. b FITC-TVRTSAE peptide binds to SKOV3 cells via the FRα. Representative immunofluorescence images show FITC-TVRTSAE peptide binding to SKOV3 cells. FITC-labelled TVRTSAEGGCGG-COOH peptide (0.5 mM) was incubated on SKOV3 cells in PBS/1% BSA for 1 h at 4 °C (upper panel). Cells treated with folic acid (0.5 mM) in PBS/1% BSA were incubated for 1 h at 4 °C prior to treatment with peptide (lower panel).

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