Fig. 6: Binding and trafficking of fluorescently labelled HAdV-C5 and HAdV-C5.KO1.TVR in SKOV3 cells. | Cancer Gene Therapy

Fig. 6: Binding and trafficking of fluorescently labelled HAdV-C5 and HAdV-C5.KO1.TVR in SKOV3 cells.

From: Identification of folate receptor α (FRα) binding oligopeptides and their evaluation for targeted virotherapy applications

Fig. 6

Cells were incubated with 250,000 vp/cell of labelled virus in serum-free medium and incubated on ice for 45 min. Cells were then transferred to 37 °C and imaged after 0, 30 or 60 min. Representative immunofluorescence images are shown. Green; Alexa Fluor 488-labelled HAdV-C5 or HAdV-C5.KO1.TVR; blue—nuclei stained with DAPI; red—actin cytoskeleton stained with Alexa Fluor 555 Phalloidin. For simplicity, HAdV-C5 is abbreviated to Ad5 in the figure.

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