Fig. 1: β-Catenin level determines the varying responses of colon cancer cells to OCA. | Cancer Gene Therapy

Fig. 1: β-Catenin level determines the varying responses of colon cancer cells to OCA.

From: Synergistic tumor inhibition of colon cancer cells by nitazoxanide and obeticholic acid, a farnesoid X receptor ligand

Fig. 1

a The effect of FXR agonist OCA on the viability of colon cancer cells SW403, SW480, DLD-1, HT-29, HCT116, and RKO detected by CCK8 assays. b The effect of OCA on the protein levels of p21CIP1, cyclin D1, c-Myc, and SHP in colon cancer cells detected by western blotting analysis. c–f The effect of OCA on the mRNA levels of p21CIP1, cyclin D1, c-Myc, and SHP in colon cancer cells detected by real-time PCR. g The effect of OCA on the luciferase activities of TOP/FOP-Flash reporter plasmid in colon cancer cells. h The expression of β-catenin in colon cancer cells detected by western blotting analysis. All data are the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01.

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