Fig. 6: Effect of EGFR, ErbB2, Gq, and MMPs inhibitors on F1-induced B-cell clonogenicity.

A, C Raji (A) and Bjab (C) cells form colonies when sorted into 96-well plates as single cell. Plates were cultured for 8 and 12 days, respectively, in the presence or absence of F1 peptide (10 ng/ml) and EGFR inhibitor AG1478 (250 nM) or ErbB2 inhibitor AG879 (2 μM) or YM-254890 (100 nM), which blocks Gq-mediated signaling. The colony area of Raji (A) and Bjab (C) was measured (15 colonies/condition) by using Leica Qwin image analysis software (left panel). B, D The same number of colonies (15 colonies/condition) was aseptically harvested from 96-well plates and stained with propidium iodide (PI) to detect PI-viable cells by flow cytometry. Absolute cell counts were obtained by the counting function of the MACSQuant® Analyzer. Bars represent the means ± SD of three independent experiments. The statistical significance between control and treated cultures was calculated using one-way ANOVA and the Bonferroni’s post-test was used to compare data. NT, not treated. ***P < 0.001.