Fig. 2: Coding transcriptome analyses of prostate cancer tumoroids. | Cancer Gene Therapy

Fig. 2: Coding transcriptome analyses of prostate cancer tumoroids.

From: CHD1 loss negatively influences metastasis-free survival in R0-resected prostate cancer patients and promotes spontaneous metastasis in vivo

Fig. 2: Coding transcriptome analyses of prostate cancer tumoroids.The alternative text for this image may have been generated using AI.

Control and CHD1-KD ARCAP-M and PC-3 cells were cultivated under 3D conditions and RNA was isolated from established tumoroids. RNA sequencing was performed and biostatistics analysis was focused on genes that were regulated in both models. The top 10 up- or down-regulated candidates are depicted in A. See Supplementary Excel files for all genes identified in the overlap and for full lists of genes regulated either in the PC-3 or ARCAP-M model. Note the down-regulation of PMEPA1 and up-regulation of RUNX3 after CHD1-KD in both models (validated by qPCR) (B). Accordingly, pSMAD2 levels are enhanced upon CHD1-KD in xenograft primary tumors of both models (C). Gene set enrichment analyses demonstrate up-regulation of cell cycle-promoting genes in the CHD1-KD while interferon response genes are enriched in the control (D). **p < 0.001; ***p < 0.0001.

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