Fig. 2: ZFN, TALEN, and CRISPR/Cas9 induced cell growth deficit and cell apoptosis in vitro.
From: The application of CRISPR/Cas9 system in cervical carcinogenesis
A–D Growth curves of ZFN, TALEN, and CRISPR/Cas9-treated S12 (A), SiHa (B), C33A (C), and HeLa (D) cells were constructed using the CCK-8 assay. E–H The apoptosis rate of S12 (E), SiHa (F), C33A (G), and HeLa (H) cells 48 h after treatment with ZFN, TALEN, and gRNA-E7-1 + Cas9 plasmids. I The colony-forming assay of SiHa and S12 cells after treatment with ZFN, TALEN, and gRNA-E7-1 + Cas9 plasmids. J Quantification of the number of colonies in S12 and SiHa cells of different treatment groups. K–L HPV16 E7/RB/CDK2/E2F1 expression of S12 (K) and SiHa (L) cells 48 h after treatment with ZFN, TALEN, and gRNA-E7-1 + Cas9 plasmids. ns, no significance; *p < 0.05; **p < 0.01; ***p < 0.001. (n = 3 replications) The experiments were repeated 3 times.
