Fig. 3: CRISPR/Cas9 inhibits S12 cell growth in vivo.
From: The application of CRISPR/Cas9 system in cervical carcinogenesis
Balb/c-nu mice were injected subcutaneously in the right flanks with 5 × 106 of S12 cells. Then, the CRISPR/Cas9 plasmids complexed with in vivo transfection reagents were injected intratumorally when the xenografts reached approximately 50 mm3. A The xenografts were measured every 6 days after treatment with gRNA-E7-1 + Cas9, gRNA-E7-2 + Cas9, gRNA-GFP + Cas9, and PBS. B The photograph of S12 xenografts in different treatment groups. C The estimated tumor size of S12 xenografts in different treatment groups. D Representative pictures of HE staining and IHC staining of HPV16 E7, Caspase-3, CD31, and PCNA in gRNA-E7-1 + Cas9, gRNA-E7-2 + Cas9, gRNA-GFP + Cas9, and PBS treated S12 xenografts. Scale bars: 50 μm. E The average necrosis area and protein expression of HPV16 E7, Caspase-3, CD31, and PCNA in different groups. **p < 0.01. (n = 4 replications).
