Fig. 4: Establishment and application of CRISPR/Cas9 system in K14-HPV16 transgenic mice.
From: The application of CRISPR/Cas9 system in cervical carcinogenesis
A The expression of mRFP was localized in the cervical epithelia of transgenic mice. Scale bars: 50 μm. B Transfection efficiency was optimized at the DNA-to-polymer ratio of 10 μg:1.0 μl, 10 μg:1.2 μl, and 10 μg:1.5 μl. The exfoliation of cervical cells was collected at 2, 4, and 6 days after vaginal transfection. C Representative HE staining and IHC staining of HPV16 E7 and p16 of gRNA-E7-1 + Cas9 treated K14-HPV16 transgenic mice at days 0,12,18, and 24. N = 3, Scale bars: 50 μm. D The cervical DNA sequencing of the gRNA-E7-1 targeted region of HPV16 E7 gene in gRNA-E7-1 + Cas9 treated K14-HPV16 transgenic mice.
