Fig. 6: Verlindamycin treatment induces frameshifting of antizymes 1 and 2.

A Schematic showing polyamine-induced frameshifting of antizyme. B Verlindamycin-induced frameshifting of antizymes 1 and 2 relative to 25 µM spermidine. Briefly, 293T cells were transfected with antizyme 1 or 2 frameshifting reporters or in-frame controls with the addition of 25 µM spermidine or verlindamycin as indicated. Percentage frameshifting (%FS) activity was determined with Dual-Glo (Promega) by obtaining firefly:renilla luciferase ratios, then dividing reporter values by in-frame control values. The background-corrected %FS activity of each compound concentration was then divided by the background-corrected %FS activity induced by 25 mM spermidine and multiplied by 100. All the experiments were performed in triplicates and a representative result is shown.