Fig. 1: Expression pattern of CFP1 in ovarian cancer tissues and ovarian cancer cells.
From: CpG-binding protein CFP1 promotes ovarian cancer cell proliferation by regulating BST2 transcription
A Immunohistochemistry for CFP1 protein expression in human ovarian cancer tissues. Scale bars, 5 and 20 μm. B Statistical analysis of CFP1 protein expression in different pathological types of 158 human ovary and ovarian cancer tissues. C Immunoblotting for CFP1 expression in ovarian cancer cells (OVCAR-3, HO8910, ES-2, SKOV3, CAVO3, and A2780), and immortalized mouse ovarian surface epithelium (IOSE). Actin was used as the loading control. D Immunoblotting for CFP1 expression levels in mouse tissues (liver, spleen, lung, kidney, uterus, muscle, and ovary). E Immunofluorescence assay for CFP1 (green) localization in ovarian cells (HO8910, ES-2, SKOV3, and A2780) and IOSE cells. Nuclei were stained with DAPI (blue). Scale bar, 20 μm. F MLN4924 inhibited tumor growth in mice. 1 × 106 A2780 cells were injected into the flank of nude mice. After tumor growth had reached roughly 200 mm3, the mice were randomly assigned to the MLN4924-treated group or control group. The single injection dose was 2 mg/kg. **P < 0.01, ***P < 0.001. The P value of tumor volume was determined using two-way ANOVA. G Immunoblotting revealing that MLN4924 inhibited CFP1 expression in vivo. A2780 tumor samples were harvested and then subjected to immunoblot analysis with the indicated antibodies. H Immunoblotting revealing that MLN4924 inhibited CFP1 expression in ovarian cancer (A2780) cells. Cells were subject to control or MLN4924 treatment and protein was subjected to immunoblot analysis with the indicated antibodies. I Immunoblotting results for ROC1 siRNA depletion efficiency and CFP1 expression in A2780 cells. J Western blot and qRT-PCR results for CUL4A siRNA depletion efficiency and CFP1 expression.
