Fig. 4: JAM-A expressing fibroblast-tumor co-cultures show enhanced reovirus infectivity.

A Human organoid-fibroblast co-cultures consisting of JAM-A negative or JAM-A positive fibroblasts were infected with R124 at MOI 10. 7 days post-infection, organoid-fibroblast co-cultures were embedded and stained for σ3 protein. Three different JAM-A negative (top panel) and JAM-A positive (bottom panel) are shown. Scale bar, 50 μm. B 2D co-cultures of KPC3 tumor cells (mCerulean) with JAM-A negative (CAF1) or JAM-A positive (CAF3) fibroblasts (mRuby) after infection with R124, jin-3, or mock control two days post-infection. Scale bar, 500 μm. C Cell viability of KPC3 tumor-fibroblast co-cultures two days post-infection. Two-way ANOVA for R124 and jin-3 MOI 10 conditions, *** = P ≤ 0.0001.