Fig. 4: KPNB1 promotes melanoma cell growth and metastasis in vivo. | Cancer Gene Therapy

Fig. 4: KPNB1 promotes melanoma cell growth and metastasis in vivo.

From: Tumor-promoting properties of karyopherin β1 in melanoma by stabilizing Ras-GTPase-activating protein SH3 domain-binding protein 1

Fig. 4

A, B The tumor volume and weight of mice were measured after KPNB1-overexpressed or silenced cells injection. C Relative protein expression of cyclin E and cyclin D1 in tumor tissues was measured by Western blot. D KPNB1 expression in tumor tissues was measured by immunohistochemistry. Scale Bar = 50 μm. E Melanoma cells with KPNB1 overexpression or silencing were injected via the tail vein into mice and the bioluminescence images were taken 7 weeks after injection. F H&E staining was used to detect lung metastases of melanoma cells. Scale Bar = 500 μm. G Number of lung nodules in H&E-stained lung sections in each group. N = 8. Data were shown as the mean ± SD. *P < 0.05 was determined by unpaired t-test and One-way ANOVA combined with Tukey’s post hoc test.

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