Fig. 3: Exogenous AATK expression inhibits cell growth and is associated with negative regulation of cell proliferation. | Cancer Gene Therapy

Fig. 3: Exogenous AATK expression inhibits cell growth and is associated with negative regulation of cell proliferation.

From: Epigenetically silenced apoptosis-associated tyrosine kinase (AATK) facilitates a decreased expression of Cyclin D1 and WEE1, phosphorylates TP53 and reduces cell proliferation in a kinase-dependent manner

Fig. 3

A Using the TREx293 cell system, growth curves of a control clone pool and an AATK expressing clone pool with doxycycline (induced) and without (uninduced) were generated. Cells were grown for eight days and counted every two days (displayed as mean of three independent experiments with SD, unpaired two-sided t-test). B Using the R2 Genomics Analysis and Visualization Platform [31], gene sets (public gene categories) negatively correlated to AATK expression in various cancer cell lines (GSE363133), breast cancer (tcgaBRCA1097), colon cancer (tcgaCOAD286), head and neck cancer (tcgaHNSC520), glioma (tcgaLGG516), lung cancer (GSE3141), as well as pancreatic cancer (tcgaPAAD178) were determined and are shown with color code. Solid: p < 0.001, striped: p < 0.01; dotted: p < 0.05. C Binding partners of AATK were detected in HEK293T cells after transfection of EYFP-tagged AATK and EYFP empty and subsequent pulldown with GFP-Trap and mass spectrometry. Enrichment of interaction partners of control samples was determined from three replicate experiments using the limma-based analysis package autonomics [77]. D Overrepresentation analysis of enriched interaction partners of AATK-EYFP vs. EYFP was performed against categories from the Kyoto Encyclopedia of Genes and Genomes [45] and Gene Ontology [46]. The contrast AATK-EYFP to EYFP displays the (sub)ontology GObp in the protein groups data sets. Data is facetted by regulation direction in the context of contrast. Significances were determined with a Fisher Exact Test.

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