Fig. 5: Influence of the 5-LO KO on cell growth and survival of HCT-116, HT-29 and U-2 OS cells. | Cancer Gene Therapy

Fig. 5: Influence of the 5-LO KO on cell growth and survival of HCT-116, HT-29 and U-2 OS cells.

From: Knock-out of 5-lipoxygenase in overexpressing tumor cells—consequences on gene expression and cellular function

Fig. 5

A Cell proliferation of the cells was assessed by counting the cells on a daily basis for 4 days. B In addition, pictures of the proliferating cells were taken on a daily basis. C Cell death analysis of the cells after treatment with actinomycin D (5, 10 nM), etoposide (20, 40 µM) or 5-fluorouracil (50 µM). DMSO treated cells were used as negative control. After 48 h the cells were stained with annexinV and propidium iodide and cell death was analyzed by flow cytometry. Data are presented as mean + SD from three independent experiments. Asterisks indicate significant changes vs. control vector treated cells. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001. ActD actinomycin D, Eto etoposide, VC empty vector control, 5-FU 5-fluorouracil.

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