Fig. 3: Histone PTMs profiling by mass spectrometry of maltonis-treated NB4 cells. | Cancer Gene Therapy

Fig. 3: Histone PTMs profiling by mass spectrometry of maltonis-treated NB4 cells.

From: Small molecule-induced epigenomic reprogramming of APL blasts leading to antiviral-like response and c-MYC downregulation

Fig. 3

A Heatmap display of histone PTMs levels in NB4 cells treated with 10 µM maltonis for 24 h. L/H (light/heavy) relative abundance ratios for differentially modified histone H3 and H4 peptides were obtained using a spike-in strategy (light channel: sample, heavy channel: spike-in standard), and were normalized on the average of the detected signal in the untreated control samples. Significant changes by multiple t-test (q value < 0.05) are indicated on the right. L/H ratios for untreated and treated samples are reported in Supplementary Table S1. B Interleaved scatter plot display of L/H ratios for untreated and maltonis-treated samples for selected PTMs. Total H3K9me3 indicates the sum of all the peptides containing K9me3 (H3K9me3K14unmod and H3K9me3K14ac). H3K4me3 could be quantified on two out of three replicates in the untreated samples.

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