Fig. 4: IGF2BP3 EV miRNA cargo affects IGF1R/Akt signaling.

a Circos Plot displays the relationship between the 137 predicted target genes and the most significantly GO biological processes and KEGG pathways (Bonferroni adj-pvalue < 0.01). b Representative western blot depicting the expression of IGF1R, PTEN and phosphorylation of Akt and mTOR in TC-71 parental cells fused with IGF2BP3pos EVs (CTR and shNC) and IGF2BP3neg EVs (#46 and #73). GAPDH and total proteins were used as loading controls. Blots signals were quantified against GAPDH and reported as the ratio of adjusted volume optical density (OD/mm2) to CTR.