Fig. 2: ADAM12 is upregulated in colon cancer cells following irradiation, yet loss of ADAM12 does not affect survival of the cells. | Cancer Gene Therapy

Fig. 2: ADAM12 is upregulated in colon cancer cells following irradiation, yet loss of ADAM12 does not affect survival of the cells.

From: ADAM12 expression is upregulated in cancer cells upon radiation and constitutes a prognostic factor in rectal cancer patients following radiotherapy

Fig. 2

A Fold change of ADAM12 mRNA levels at different timepoints after irradiation with 10 Gy compared to 0 Gy in SW480 cells, measured by qPCR (n = 4). B Fold change of ADAM12 mRNA levels at different timepoints after irradiation with 5 Gy compared to 0 Gy in CT26, measured by qPCR. C Fold change of cell surface ADAM12 protein levels measured by flow cytometry of CT26 cells 24 h after irradiation with 5 Gy compared to 0 Gy. D, E Validation of ADAM12-knockout using qPCR in SW480 cells (D) and CT26 cells (E). F, G Cells’ relative ability to form colonies following loss of ADAM12 in SW480 (n = 4) (F) and CT26 cells (G). H, I Clonogenic assay of WT compared to A12−/− following irradiation in SW480 (n = 4) (H) and CT26 cells (I). Repeated measures one-way ANOVA with Geisser–Greenhouse correction and correction for multiple comparisons using FDR control by Benjamini, Krieger and Yekutieli method (A, B, D–G), two-sided Welch’s t-test (C) or extra sum-of-squares F-test of linear quadratic cell death models (H, I) were applied to test for significant differences: *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001. n = 3 unless otherwise specified.

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