Fig. 4: ZDHHC1 interacts with LIPG in CRC cells.
From: ZDHHC1 downregulates LIPG and inhibits colorectal cancer growth via IGF2BP1 Palmitoylation
Cells with shControl, shZDHHC1, shLIPG, and shZDHHC1/shLIPG were harvested for western blotting and RT-qPCR analysis (A, B). Proliferation, colony formation assay and transwell invasion with HCT116 and SW480 cells were performed in each group (C–F). HCT116 cells were harvested and injected into nude mice for xenografts assay (G). Tumor volume (H) and the weight (I) were calculated, and all date are shown as mean ± SD with five replicates. LD were visualized with Bodipy 493/503 staining (green) (J). Scale bar, 40 μm. Intracellular TAG levels were quantified with the triglyceride quantification assay kit (K). Data presented as mean ± SD with three replicates. Student’s t-test and one-way ANOVA were used to determine the statistical significance. *p < 0.05; **p < 0.01; ***p < 0.001.
