Fig. 6: ZDHHC1 is a IGF2BP1-palmitoylating enzyme that induces IGF2BP1-C337 S-palmitoylation.
From: ZDHHC1 downregulates LIPG and inhibits colorectal cancer growth via IGF2BP1 Palmitoylation
ZDHHC1 could not regulate the protein and mRNA level of IGF2BP1 by western blotting and RT-qPCR analysis (A, B). IP-ABE analysis of IGF2BP1 palmitoylation in HCT116 cells when treated with or without palmitoylation inhibitor 2-BP (C, D). Predicted cysteine residues on IGF2BP1 susceptible to S-palmitoylation (E). IP-ABE analysis of IGF2BP1 palmitoylation in HCT116 cells when ectopically expressing IGF2BP1-WT or harboring specific mutations (F). Alignment of the similarity of PCSK9 sequences in different vertebrate orthologs (G). Flga-ZDHHC1 reduce the level of LIPG with IGF2BP1-WT but not IGF2BP1-C337S by western blotting analysis (H). RIP-qPCR analysis showing the enrichment of LIPG mRNA in anti-IGF2BP1 precipitates (I). MeRIP-qPCR analysis showing the m6A enrichment of LIPG mRNA in HCT116 cells (J). Data presented as mean ± SD with three replicates. Student’s t-test and one-way ANOVA were used to determine the statistical significance. *p < 0.05; **p < 0.01; ***p < 0.001.
