Fig. 1: PML-RARα degradation and ROS upregulation are insufficient to cause APL cell death. | Cancer Gene Therapy

Fig. 1: PML-RARα degradation and ROS upregulation are insufficient to cause APL cell death.

From: Arsenic trioxide and p97 inhibitor synergize against acute myeloid leukemia by targeting nascent polypeptides and activating the ZAKα–JNK pathway

Fig. 1

A NB4 cells were treated with different doses of ATO for 24 h, and apoptosis was detected by flow cytometry. B PML-RARα in the NB4 cells was detected by immunoblot after treating with different doses of ATO for 12 h. C ROS level in NB4 induced by ATO for 4 h was detected by using 10 μM DCFH-DA. D Quantification of ROS level in NB4 induced by ATO for 4 h. Normalized to control (0 μM ATO). E NB4 cells were treated with 1.5 μM ATO, 10 or 20 μM H2O2 for 4 h, and then ROS level was detected by using 10 μM DCFH-DA. F Quantification of ROS level in NB4 induced by 1.5 μM ATO, 10 or 20 μM H2O2 for 4 h. Normalized to control. G Quantification of ROS level in NB4 induced by 1.5 μM ATO, 10 or 20 μM H2O2 for 24 h. Normalized to control. H The apoptosis ratio of NB4 cells treated with 1.5 μM ATO or different doses of H2O2 for 24 h was detected by flow cytometry. I NB4 cells were pre-treated with 8 mM NAC or 10 mM GSH for 1 h, and then 2 μM ATO was added for another 24 h. Apoptosis was detected by flow cytometry. Two-tailed Student t test, **P < 0.01, ***P < 0.001, ****P < 0.0001, the ns indicate no significant difference. Error bars reflect ± SEM of three independent experiments.

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