Table 1 Various sense strand sequences for expressing shRNAs.

From: Combined downregulation of TGF-β1 and GRP78 is responsible for overcoming acquired sorafenib resistance, which is initiated by rewiring the cell surface CD44-GRP78-IGF-1R signaling circuit

Name

Seqeunce (5’-3’)

U6-shGRP78 1st

GATCCGAGGTTACCCATGCAGTTGTTTCTCAACAACTGCATGGGTAACCTTTTTTTTA

U6-shGRP78 2nd

GATCCGGTTACCCATGCAGTTGTTACTCTCGTAACAACTGCATGGGTAACCTTTTA

H1-shGRP78 3rd (GRP78 shRNA 5 of Genolution)

GATCCCCAAGATGCTGACATTGAAGATCTCTCTTCAATGTCAGCATCTTGGTTTTA

H1-shGRP78 4th (GRP78 shRNA 5 of Genolution)

GATCCCCAAGATGCTGACATTGAAGATCTCTCTCTTCAATGTCAGCATCTTGGTTTTTTA

H1-shGRP78 5th

GATCCGGTTACCCATGCAGTTGTTACTCTCTCGTAACAACTGCATGGGTAACCTTTTTTA

  1. The 2nd and 5th sequences are the same target sequences but different promoters and different loop sequences and poly A sequences, while 3rd and 4th sequences are the same target sequences with the same promoter but different loop sequences and poly A sequences.
Back to article page