Fig. 2: Macropinocytosis-mediated potentiation of sensitivity to adenoviruses in PSCs incubated with PDAC-CM. | Cancer Gene Therapy

Fig. 2: Macropinocytosis-mediated potentiation of sensitivity to adenoviruses in PSCs incubated with PDAC-CM.

From: p53-armed oncolytic adenovirus induces apoptosis in pancreatic cancer-associated stellate cells via macropinocytosis

Fig. 2

A Representative images of TMR-dextran accumulation by hPSC-5 cells incubated in the presence of SFM or PDAC-CM. Scale bars: 200 μm. B hPSC-5 cells were treated with DMSO or EIPA in the presence of TMR-dextran in SFM or PDAC-CM, and the dextran index was calculated using ImageJ software. DMSO was used as the vehicle control. C Quantitative measurement of copy number of viral E1A DNA in hPSC-5 cells treated with DMSO or EIPA and infected with Ad5 (MOI 25) for 2 h in the presence of SFM or PDAC-CM. D Representative images of GFP expression in hPSC-5 cells treated with DMSO or EIPA and infected with Ad-GFP (MOI 100) for 48 h in the presence of SFM or PDAC-CM. Scale bars: 200 μm. E Relative fluorescence intensity of GFP was analyzed using ImageJ software. Data are expressed as mean ± SD (n = 3). The statistical significance of differences between two groups or three groups was determined using the two-tailed, non-paired Student’s t-test or two-tailed one-way ANOVA followed by Tukey’s comparison tests, respectively. N.S., not significant; *, P < 0.05; **, P < 0.01; ****, P < 0.0001 (vs DMSO or SFM).

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