Fig. 3: Macropinocytosis-mediated potentiation of sensitivity to oncolytic adenoviruses in PSCs incubated with PDAC-CM. | Cancer Gene Therapy

Fig. 3: Macropinocytosis-mediated potentiation of sensitivity to oncolytic adenoviruses in PSCs incubated with PDAC-CM.

From: p53-armed oncolytic adenovirus induces apoptosis in pancreatic cancer-associated stellate cells via macropinocytosis

Fig. 3

A Schematic diagrams of structures of the oncolytic adenoviruses. OBP-301 is a telomerase-specific, replication-competent oncolytic adenovirus in which the hTERT promoter drives expression of the adenoviral E1A and E1B genes, which are linked by an IRES. OBP-401 is a GFP-expressing OBP-301 variant generated by inserting the CMV promoter-driven GFP gene expression cassette into the E3 region of OBP-301. OBP-702 is a p53-armed OBP-301 variant generated by inserting the Egr1 promoter-driven human wild-type p53 gene expression cassette into the E3 region of OBP-301. B Representative images of GFP fluorescence in hPSC-5 cells following infection with OBP-401 (MOI 100) for 48 h in the presence of SFM or PDAC-CM. Scale bars: 200 μm. C Relative fluorescence intensity of GFP was analyzed by ImageJ software. D Representative images of GFP fluorescence in hPSC-5 cells treated with DMSO or EIPA and infected with OBP-401 (MOI 100) for 48 h in the presence of SFM or PDAC-CM. Scale bars: 200 μm. E Relative fluorescence intensity of GFP was analyzed using ImageJ software. F, G Quantitative measurement of copy number of viral E1A DNA in hPSC-5 cells treated with DMSO or EIPA and infected with OBP-301 (MOI 25) (F) or OBP-702 (MOI 25) (G) for 2 h in the presence of SFM or PDAC-CM. Data are expressed as mean ± SD (n = 3). The statistical significance of differences between two groups or three groups was determined using the two-tailed, non-paired Student’s t-test or two-tailed one-way ANOVA followed by Tukey’s comparison tests, respectively. N.S., not significant; *, P < 0.05; ***, P < 0.001; ****, P < 0.0001.

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