Fig. 7: Neutrophil recruitment upon UVB irradiation is not dependent on Langerhans cells | Cell Death & Disease

Fig. 7: Neutrophil recruitment upon UVB irradiation is not dependent on Langerhans cells

From: Expression of inflammasome proteins and inflammasome activation occurs in human, but not in murine keratinocytes

Fig. 7

a Experimental setup of depletion of Langerhans cells in Lang-DTREGPF mice using diphtheria toxin (18 ng/g body weight). Control animals were injected with vehicle. After 4 days, animals were subjected to UVB irradiation (300 mJ/cm2), non-irradiated animals served as control. b Depletion of CD207+ Langerhans cells was controlled by multicolour flow cytometry. c The percentage of CD45+ CD11b+ Ly-6G+ neutrophils and of CD45+ CD64hi Ly-6Chi pro-inflammatory macrophages was determined by flow cytometry. Error bars represent mean ± SD of a representative experiment with n = 5 animals/group. One-way ANOVA with Sidak’s multiple comparison was performed (****p < 0.0001, **p < 0.01). ns not significant, DT diphtheria toxin

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