Fig. 2: Effect of 400 μM H2O2 treatment on A6 cells during recovery time

a Histograms of cell viability assayed by trypan blue exclusion assays: treated cells '24 h' with 400 μM H2O2 and after 1, 2, 5, 6, 7 and 8 days of recovery (R) calculated respect to 24 h. b Histograms of cell cycle phase distribution: control cells 'C', treated cells (400 μM H2O2 24 h) and after 1, 2, 5 and 8 days of recovery are represented. c Histograms of ROS content: control cells “C”, treated cells (400 μM H2O2 24 h) and after 1, 2, 5, 6, 7 and 8 days of recovery are represented. The ROS values of samples were reported in graph as multiple of cell control value (see methods). All data were obtained from three independent experiments. Error bars indicate standard deviation. *P < 0.5, **P < 0.05, ***P < 0.005 vs control group, or H2O2 group