Fig. 1

Oridonin induced apoptosis in human osteosarcoma cells. a Structure of oridonin (C₂₀H₂₈O₆, MW = 364.4327). b The effect of oridonin on the cell viability of MG-63, HOS, Saos-2 and U-2OS cells after 24 h treatment. c The effect of oridonin on the cell viability of MG-63, HOS, Saos-2, and U-2OS cells after 48 h treatment. d The effect of oridonin on the cell viability of human normal cells L02, HUVEC, HEK-293 and human normal osteogenic cell lines hFOB 1.19 after 24 h treatment. e After given oridonin (5, 10, and 15 µM) for 24 h, the nuclear morphologic changes of MG-63 and HOS cells were observed by fluorescent microscope (400×). Apoptosis bodies and nuclei pyknosis were observed in apoptosis cells. f, g The apoptotic rates of cells induced by oridonin in MG-63 and HOS cells detected by Annexin V/PI double-staining assay. The results are representative of at least three independent experiments and shown as mean ± SD. **P < 0.01 compared with control group