Fig. 5
From: Oridonin exerts anticancer effect on osteosarcoma by activating PPAR-γ and inhibiting Nrf2 pathway
Oridonin induced apoptosis by triggering ROS generation via inhibiting Nrf2 signaling pathway in vitro. a-e MG-63 and HOS cells were transfected with Nrf2 plamid for 8 h followed by with/without 15 µM oridonin for 24 h. a The protein expression of Nrf2, NQO1, HO-1 in MG-63 and HOS cells were determined by Western blot analysis. β-actin was used as the loading control. b Gray scale analysis was performed to determine the relative ratios of Nrf2, NQO1, HO-1. The results are shown as means ± SD from three independent experiments. **P < 0.01 compared with control group; ##P < 0.01 compared with 15 µM oridonin group. c, d The mRNA levels of NQO1, HO-1 in MG-63 and HOS cells were measured by real-time PCR. The results are shown as means ± SD from three independent experiments. **P < 0.01 compared with control group; ##P < 0.01 compared with 15 µM oridonin group. e The level of intracellular ROS was measured by flow cytometry. The results are shown as means ± SD from three independent experiments. **P < 0.01 compared with control group; ##P < 0.01 compared with 15 µM oridonin group. f The respective apoptosis rates of different groups were measured by Annexin V/PI staining. The results are representative of at least three independent experiments and shown as mean ± SD. **P < 0.01 compared with control group; ##P < 0.01 compared with 15 µM oridonin group
