Fig. 8: Effects of FEZF1-AS1 or ZNF312B knockdown on the inhibition of PDAC cell glycolytic capacity could be rescued by miR-107 inhibitor in vitro | Cell Death & Disease

Fig. 8: Effects of FEZF1-AS1 or ZNF312B knockdown on the inhibition of PDAC cell glycolytic capacity could be rescued by miR-107 inhibitor in vitro

From: FEZF1-AS1/miR-107/ZNF312B axis facilitates progression and Warburg effect in pancreatic ductal adenocarcinoma

Fig. 8

a, b Silencing of FEZF1-AS1 expression abrogated the glycolytic capacity of PANC-1 and Capan-2 cells, while the miR-107 inhibitor could rescue the inhibitory effects of si-FEZF1-AS1 on the glycolytic process in both PDAC cell lines, as reflected by ECAR analysis. c, d Silencing of ZNF312B expression inhibited the glycolytic capacity in PANC-1 and Capan-2 cells, as reflected by ECAR analysis. e, f Silencing of FEZF1-AS1 or ZNF312B expression inhibited the glucose uptake and lactate production in PANC-1 and Capan-2 cells, while the miR-107 inhibitor could rescued the effect of si-FEZF1-AS1 or si-ZNF312B on glucose uptake and lactate production of both PDAC cell lines. Intracellular glucose levels were measured and normalized based on protein concentration. g PANC-1 and Capan-2 cells-expressing si-FEZF1-AS1 or si-ZNF312B combined with miR-107 inhibitor were cultured under normoxic conditions for 24 h. Acidification of the culture medium was evaluated by visually inspecting the colour of the medium. *P < 0.05, **P < 0.01

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