Fig. 6: The transcriptional repression of DUSP6 by FBXL10 leads to ERK1/2 activation and facilitates the proliferation of DLBCL cells

a Effect of FBXL10 knockdown on phosphorylation level of ERK1/2. OCI-Ly1 cells transduced with shControl or shFBXL10#1 or #2 virus were examined by Western blot analysis with indicated antibodies. b Effect of FBXL10 knockdown on phospho-ERK1/2 levels during serum activation. FBXL10 depleted OCI-Ly1 cells and control cells were stimulated with 10% serum for 5, 15, or 30 min after 18 h of serum starvation. Protein extracts were then examined by Western blot analysis. c Effect of double knockdown of FBXL10 and DUSP6 on phosphorylation levels of ERK1/2 during serum activation. Control, FBXL10-depleted, and DUSP6/FBXL10-depleted OCI-Ly1 cells were stimulated with 10% serum for 30 min. For a–c, β-actin served as a loading control for total protein. d Rescue of the proliferation defect of FBXL10-depleted OCI-Ly1 cells by DUSP6 knockdown. The cell numbers were determined at 1–4 days using trypan blue staining for the indicated groups. The error bars denote S.E.M., n = 3