Fig. 1: The Inhibition of mitochondrial glutamine metabolism induces senescence in PDAC cells | Cell Death & Disease

Fig. 1: The Inhibition of mitochondrial glutamine metabolism induces senescence in PDAC cells

From: Mitochondrial glutamine metabolism via GOT2 supports pancreatic cancer growth through senescence inhibition

Fig. 1

a, 8988T cells were plated in complete media which was replaced the following day with Gln-free medium. Percentages of SA-β-gal positive cells are shown. b, Representative images of SA β-gal staining for 8988T cells in the presence or absence of Gln. c, Percentages of SA-β-gal positive cells in 8988T cells treated with increasing concentrations of DON. d, Percentages of SA-β-gal positive cells in control (shGFP) and GLS knockdown (shGLS) 8988T cells (left). Western blot confirmed knockdown of GLS expression (right). β-actin serves as a loading control. All error bars ± SEM. **p < 0.01 and ***p < 0.001

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