Fig. 5: p27 is responsible for GOT2 knockdown-mediated senescence induction in PDAC cells | Cell Death & Disease

Fig. 5: p27 is responsible for GOT2 knockdown-mediated senescence induction in PDAC cells

From: Mitochondrial glutamine metabolism via GOT2 supports pancreatic cancer growth through senescence inhibition

Fig. 5

a, Percentages of SA-β-gal positives cells in 8988T cells expressing a control shRNA or two independent shRNAs to GOT2 supplemented with or without PFT-α. b, Relative mRNA levels of indicated genes in 8988T cells expressing a control shRNA or a shRNA to GOT2. c, p21 and p53 protein levels in whole-cell lysates from 8988T cells expressing a control shRNA or shRNAs to GOT2. β-actin serves as a loading control. d, The effect of GOT2 knockdown on p27 protein levels in 8988T cells expressing a control shRNA or shRNAs to GOT2. e and f, p27 protein levels in 8988T cells expressing a control shRNA or a shRNA to GOT2 supplemented with or without NAC e, OAA or Asp f. g, Percentages of SA-β-gal positive cells in control and GOT2 knockdown 8988T cells transfected with p27 or control siRNA as indicated. h, A proposed model depicting the regulation of PDAC senescence by GOT2. All error bars ± SEM. **p < 0.01 and ***p < 0.001

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