Fig. 6: IL-2-mediated glycolysis promotes the localization and cleavage of caspase-3 in lipid rafts

Activated T cells were cultured for 3 days in IL-2 or IL-15. a, b Cells were sonicated before fractionation in an ultracentrifuge over an Optiprep density gradient. Fractions of 1 mL each were collected. A volume of 30 μL of each fraction was used to perform an immunoblot for capsase-3, GM-1 (lipid rafts), and paxillin (non-raft; the blot is representative of two independent experiments). c Densitometry of the ratio of cleaved capsase-3 to pro-caspase-3 in raft and non-raft fractions (n = 2 independent experiments). d bVAD precipitation of active caspases was performed on the indicated raft and non-raft fractions, and precipitates were immunoblotted for active caspase-3 (the blot is representative of two independent experiments). e Densitometry of active caspase-3 in raft and non-raft fractions (n = 2 independent experiments)