Fig. 3: miR-4779 expression and cell viability by miR-4779 inhibitor are higher in normal cell lines than cancer cell lines | Cell Death & Disease

Fig. 3: miR-4779 expression and cell viability by miR-4779 inhibitor are higher in normal cell lines than cancer cell lines

From: MicroRNA miR-4779 suppresses tumor growth by inducing apoptosis and cell cycle arrest through direct targeting of PAK2 and CCND3

Fig. 3

a–c HCT116 cells were transfected with mimics or inhibitors of miR-NC and miR-4779. At 24 h after transfection, mRNA expression levels of PAK2 and CCND3 were determined by qRT-PCR a. At 48 h after transfection, protein levels of PAK2 and CCND3 were determined by western blotting using the corresponding antibodies b. At 72 h after transfection, cell viability was determined by MTS assay c. d Comparison of miR-4779 expression in cancer and normal cells. Expression of mature miR-4779 was determined by qRT-PCR. e Normal cell lines CCD-18Co and BEAS-2B were transfected with mimics or inhibitors of miR-NC and miR-4779. At 72 h after transfection, cell viability was determined by MTS assay. f Normal cell lines CCD-18Co and BEAS-2B were transfected with miR-NC or miR-4779 for 24 h. The expression of mature miR-4779 was determined by qRT-PCR. All data are presented as averages of triplicate measurements with error bars representing standard deviations. **P < 0.01 and ***P < 0.001

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