Fig. 3: N-terminal domain of FasL is important for interaction with caveolin-1 | Cell Death & Disease

Fig. 3: N-terminal domain of FasL is important for interaction with caveolin-1

From: Impairment of Fas-ligand–caveolin-1 interaction inhibits Fas-ligand translocation to rafts and Fas-ligand-induced cell death

Fig. 3

a Schematic diagram of FasL and its mutant forms. PRD polyproline domain, TM transmembrane domain. b Immunoblot analysis of FasL and caveolin-1 expression in HeLa cells transfected with wild (wt) and mutant forms of FasL (form I: Δ1–42, form II: Δ43–63, and form III: Δ64–80). c HeLa cells overexpressing wild type (wt) and mutant forms (Δ1–42, Δ43–63, and Δ64–80) of FasL were lysed, and immunoprecipitation with anti-caveolin-1 Abs was carried out followed by staining with anti-Fas-ligand mAbs (upper panel), or lysates were immunoprecipitated with anti-Fas-ligand mAbs followed by staining with anti-caveolin-1 Abs (lower panel)

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