Fig. 1: Induction of necrosis in ovarian cancer cells

a Expression of RIPK1, RIPK3, caspase-8 and MLKL was assessed in ovarian cancer cells and HeLa by immunoblot. b OVCAR4, TOV21G and HeLa cells were treated with TNF-α (T, 20 ng/ml), the Smac-mimetic LCL-161 (S, 1 μM) and/or zVAD.fmk (Z, 25 µM) for 6 h in the presence and absence of necrostatin-1 (Nec, 10 µM) and necrosulfonamide (NSA, 1 µM). Arrows indicate necrosis induced by TSZ in TOV21G cells, reversed by Nec and NSA. Cell survival was assessed by MTT assay. c OVCAR4, TOV21G and HeLa cells were infected with dl922-947, Ad5, Ad35 and Ad11p (MOI 0.01–1000 pfu/cell) for 120 h. Cell survival was assessed by MTT assay. d Transmission electron microscopic images of TOV21G cells following 6 h treatment with TSZ (concentrations as 1B) or 48 h infection with dl922-947 (MOI 1). White arrows indicate sites of membrane rupture; black arrows indicate electron-dense mitochondria