Fig. 2: HOTTIP was upregulated in SCLC drug-resistant cell lines and establishment of HOTTIP overexpression and knockdown cell lines | Cell Death & Disease

Fig. 2: HOTTIP was upregulated in SCLC drug-resistant cell lines and establishment of HOTTIP overexpression and knockdown cell lines

From: Long non-coding RNA HOTTIP promotes BCL-2 expression and induces chemoresistance in small cell lung cancer by sponging miR-216a

Fig. 2

a Expression level of HOTTIP in four types of SCLC and 16-HBE cell lines show differential expression in SCLC cell lines, especially in drug-resistant cell lines, compared with expression in 16-HBE cell line. b Establishment of HOTTIP overexpression cell lines with a pcDNA3.1-HOTTIP expression vector being transfected into H69 and H446 cells, respectively. c Establishment of HOTTIP knockdown cell lines by siRNA method in H69AR and H446AR cells, respectively. d For stable HOTTIP RNAi effects, the RNAi sequences of si-HOTTIP-1 and si-HOTTIP-2 combination were packaged by lentivirus for the following studies, the transfection effects above were validated by electron fluorescence microscopy technique and RT-qPCR. *P < 0.05; **P < 0.001

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